J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, W. B.
Right arrow Articles by Won Choe, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, W. B.
Right arrow Articles by Won Choe, K.
Agricola
Right arrow Articles by Park, W. B.
Right arrow Articles by Won Choe, K.
J Med Microbiol 56 (2007), 1290-1295; DOI: 10.1099/jmm.0.46765-0
© 2007 Society for General Microbiology
ISSN 1473-5644

In vitro ability of Staphylococcus aureus isolates from bacteraemic patients with and without metastatic complications to invade vascular endothelial cells

Wan Beom Park1, Sung Han Kim1, Cheol-in Kang1, Jae Hyun Cho1, Ji Whan Bang1, Kyoung Wha Park1,{dagger}, Yeong Seon Lee2, Nam Joong Kim1, Myoung-don Oh1, Hong Bin Kim1,{ddagger} and Kang Won Choe1

1 Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea

2 National Institute of Health, Korea Center for Diseases Control and Prevention, Seoul 122-701, Republic of Korea

Correspondence
Hong Bin Kim
hbkimmd{at}snu.ac.kr

Received 7 June 2006
Accepted 25 June 2007

Invasion of vascular endothelial cells is thought to be a critical step in the development of metastatic infections in patients with Staphylococcus aureus bacteraemia. This study was designed to evaluate the association between the ability to invade endothelial cells and metastatic infection by S. aureus. Patients with metastatic infection were identified among those with community-acquired S. aureus bacteraemia in a tertiary referral hospital. Patients with simple bacteraemia caused by S. aureus over the same period served as the control group. The ability of each clinical isolate to invade endothelial cells was evaluated by counting the number of intracellular organisms 1 h after inoculation onto human umbilical vein endothelial cells in vitro. The cytotoxic activity of intracellular S. aureus was determined 24 h after internalization, and expressed as the percentage of cells killed. The clinical isolates varied in invasiveness and cytotoxicity. The median invasiveness, relative to S. aureus reference strain ATCC 29213, was 145 % in the cases (n=10) [interquartile range (IQR) 103–160] and 153 % (IQR 111–173) in the controls (n=11; P=0.44). The median cytotoxicity was 59.4 % (IQR 47–68) in the cases and 65.2 % (IQR 50–74) in the controls (P=0.44). Differences in the ability of S. aureus to invade and destroy vascular endothelial cells in vitro were not associated with the development of metastatic complications in patients with S. aureus bacteraemia. This implies that the invasiveness and toxicity of S. aureus for endothelial cells may not be major determinants of metastatic infection.

Abbreviations: HUVEC, human umbilical vein endothelial cell; IQR, interquartile range.

{dagger}Present address: Department of Internal Medicine, Chonnam National University Hospital, Gwangju 501-757, Republic of Korea.

{ddagger}Present address: Department of Internal Medicine, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-707, Republic of Korea.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.