J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Milne, T. S.
Right arrow Articles by Titball, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Milne, T. S.
Right arrow Articles by Titball, R. W.
Agricola
Right arrow Articles by Milne, T. S.
Right arrow Articles by Titball, R. W.
J Med Microbiol 56 (2007), 1268-1276; DOI: 10.1099/jmm.0.47190-0
© 2007 Society for General Microbiology
ISSN 1473-5644

A 55 kDa hypothetical membrane protein is an iron-regulated virulence factor of Francisella tularensis subsp. novicida U112

Timothy S. Milne1, Stephen L. Michell1,{dagger}, Helen Diaper1, Per Wikström2, Kerstin Svensson2, Petra C. F. Oyston1,3 and Richard W. Titball1,4,{dagger}

1 Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK

2 Swedish Defence Research Agency, SE-901 82 Umeå, Sweden

3 Department of Infection, Immunity and Inflammation, Maurice Shock Building, University of Leicester, PO Box 138, Leicester LE1 9HN, UK

4 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK

Correspondence
Timothy S. Milne
TSMILNE{at}dstl.gov.uk

Received 26 January 2007
Accepted 13 June 2007

Iron is an important nutritional requirement for bacteria due to its conserved role in many essential metabolic processes. As a consequence of the lack of freely available iron in the mammalian host, bacteria upregulate a range of virulence factors during infection. Transcriptional analysis of Francisella tularensis subsp. novicida U112 grown in iron-deficient medium identified 21 genes upregulated in response to this condition, four of which were attributed to a siderophore operon. In addition, a novel iron-regulated gene, FTT0025, was identified which is part of this operon and encodes a 55 kDa hypothetical membrane protein. When grown on chrome azurol S agar, the F. tularensis subsp. novicida U112{Delta}FTT0025 mutant produced an increased reaction zone compared with the wild-type, suggesting that siderophore production was unaffected but that the bacteria may have a deficiency in their ability to re-sequester this iron-binding molecule. Furthermore, the {Delta}FTT0025 mutant was attenuated in a BALB/c mouse model of infection relative to wild-type F. tularensis subsp. novicida U112.

Abbreviations: CAS, chrome azurol S; LVS, live vaccine strain; MLD, median lethal dose; s.c., subcutaneous.

{dagger}Present address: School of Biosciences, Geoffrey Pope Building, University of Exeter, Exeter EX4 4QD, UK.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
H. Lindgren, M. Honn, I. Golovlev, K. Kadzhaev, W. Conlan, and A. Sjostedt
The 58-Kilodalton Major Virulence Factor of Francisella tularensis Is Required for Efficient Utilization of Iron
Infect. Immun., October 1, 2009; 77(10): 4429 - 4436.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. A. Allard, J. Dao, P. Sanjeevaiah, K. McCoy-Simandle, C. H. Chatfield, D. S. Crumrine, D. Castignetti, and N. P. Cianciotto
Purification of Legiobactin and Importance of This Siderophore in Lung Infection by Legionella pneumophila
Infect. Immun., July 1, 2009; 77(7): 2887 - 2895.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. W. Buchan, R. L. McCaffrey, S. R. Lindemann, L.-A. H. Allen, and B. D. Jones
Identification of migR, a Regulatory Element of the Francisella tularensis Live Vaccine Strain iglABCD Virulence Operon Required for Normal Replication and Trafficking in Macrophages
Infect. Immun., June 1, 2009; 77(6): 2517 - 2529.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
M. I. Richards, S. L. Michell, and P. C. F. Oyston
An intracellularly inducible gene involved in virulence and polyphosphate production in Francisella
J. Med. Microbiol., October 1, 2008; 57(10): 1183 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
P. C. F. Oyston
Francisella tularensis: unravelling the secrets of an intracellular pathogen
J. Med. Microbiol., August 1, 2008; 57(8): 921 - 930.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.