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J Med Microbiol 56 (2007), 77-82; DOI: 10.1099/jmm.0.46810-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface

Mayumi Ueta1, Tetsuya Iida2, Masako Sakamoto2, Chie Sotozono1, Junko Takahashi1, Kentaro Kojima1, Kazuhisa Okada2, Xiuhao Chen2, Shigeru Kinoshita1 and Takeshi Honda2

1 Department of Ophthalmology, Kyoto Prefectural University of Medicine, Hirokoji, Kawaramachi, Kamigyoku, Kyoto 602-0841, Japan

2 Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka, Japan

Correspondence
Mayumi Ueta
mueta{at}ophth.kpu-m.ac.jp

Received 2 July 2006
Accepted 14 September 2006

Staphylococcus epidermidis is part of the normal bacterial flora on the ocular surface. The chromosomal DNA of bacterial isolates obtained from the conjunctival sac, upper and lower lid margins, and upper and lower Meibomian glands of healthy volunteers was subjected to SmaI digestion and PFGE to study the genetic diversity of the organisms. Multiple colonies were also examined of S. epidermidis derived from the conjunctival sac of the same subjects. Lastly, commensal bacteria were harvested from the ocular surfaces of four healthy subjects once a month for 6 months, and the genetic background of the S. epidermidis isolates was analysed. It was found that bacterial strains not only from different subjects but also from multiple ocular surface sites of the same subject exhibited different PFGE patterns. In five of 42 subjects multiple colonies of S. epidermidis were isolated from the conjunctival sac; three harboured multiple colonies with different PFGE patterns, and two manifested multiple colonies with identical PFGE patterns. S. epidermidis isolated from the conjunctival sac of the same subjects over a 6-month period exhibited varying PFGE patterns. The data demonstrate the polyclonality of S. epidermidis on the healthy ocular surface.




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