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J Med Microbiol 56 (2007), 52-55; DOI: 10.1099/jmm.0.46909-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum ß-lactamases

Christopher I. Birkett1, Hugo A. Ludlam1, Neil Woodford2, Derek F. J. Brown1, Nicholas M. Brown1, Mark T. M. Roberts1, Nicola Milner1 and Martin D. Curran1

1 Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, UK

2 Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London, UK

Correspondence
Christopher I. Birkett
christopher.birkett{at}addenbrookes.nhs.uk

Received 22 August 2006
Accepted 22 September 2006

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type blaCTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on ß-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum ß-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed blaCTX-M genes in 21 of 28 ESBL-producing isolates.

Abbreviations: ESBL, extended-spectrum ß-lactamase.




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