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1 Department of Periodontics and Endodontics, University at Buffalo School of Dentistry, Buffalo, NY, USA
2 Department of Oral Biology, University at Buffalo School of Dentistry, Buffalo, NY, USA
Correspondence
M. M. Vickerman
mmv4{at}buffalo.edu
Received 14 July 2006
Accepted 24 August 2006
750 clones amplified from each patient group with universal bacterial primers were matched to the Ribosomal Database Project II database. Phylotypes from 37 genera representing Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria were identified. Results were compared to those obtained with species-specific primers designed to detect Prevotella intermedia, Porphyromonas gingivalis, Porphyromonas endodontalis, Peptostreptococcus micros, Enterococcus sp., Streptococcus sp., Fusobacterium nucleatum, Tannerella forsythensis and Treponema denticola. Since members of the domain Archaea have been implicated in the severity of periodontal disease, and a recent report confirms that archaea are present in endodontic infections, 16S archaeal primers were also used to detect which patients carried these prokaryotes, to determine if their presence correlated with severity of the clinical symptoms. A Methanobrevibacter oralis-like species was detected in one asymptomatic and one symptomatic patient. DNA from root canals of these two patients was further analysed using species-specific primers to determine bacterial cohabitants. Trep. denticola was detected in the asymptomatic but not the symptomatic patient. Conversely, Porph. endodontalis was found in the symptomatic but not the asymptomatic patient. All other species except enterococci were detected with the species-specific primers in both patients. These results confirm the presence of archaea in root canals and provide additional insights into the polymicrobial communities in endodontic infections associated with clinical symptoms.
Abbreviations: RDP-II, Ribosomal Database Project II.
The GenBank/EMBL/DDBJ Trace Archive accession numbers for the bacterial 16S rDNA sequences are 1391568375–1391571307, and for the archaeal 16S rDNA sequences 1391568038–1391568374 and 1391557217–1391557263.
A table showing the taxonomic distribution of bacterial phylotypes detected in the study is available as supplementary data with the online version of this paper.
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