J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rehm, T.
Right arrow Articles by Goethe, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rehm, T.
Right arrow Articles by Goethe, R.
Agricola
Right arrow Articles by Rehm, T.
Right arrow Articles by Goethe, R.
J Med Microbiol 56 (2007), 102-109; DOI: 10.1099/jmm.0.46616-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Amplified fragment length polymorphism of Streptococcus suis strains correlates with their profile of virulence-associated genes and clinical background

Thomas Rehm1, Christoph G. Baums1, Birgit Strommenger2, Martin Beyerbach3, Peter Valentin-Weigand1 and Ralph Goethe1

1 Institut für Mikrobiologie, Zentrum für Infektionsmedizin, Stiftung Tierärztliche Hochschule Hannover, D-30173 Hannover, Germany

2 Robert Koch Institut, Wernigerode Branch, D-38855 Wernigerode, Germany

3 Institut für Biometrie, Epidemiologie und Informationsverarbeitung, Stiftung Tierärztliche Hochschule Hannover, D-30173 Hannover, Germany

Correspondence
Christoph G. Baums
christoph.baums{at}gmx.de

Received 8 March 2006
Accepted 29 September 2006

Amplified fragment length polymorphism (AFLP) typing was applied to 116 Streptococcus suis isolates with different clinical backgrounds (invasive/pneumonia/carrier/human) and with known profiles of virulence-associated genes (cps1, -2, -7 and -9, as well as mrp, epf and sly). A dendrogram was generated that allowed identification of two clusters (A and C) with different subclusters (A1, A2, C1 and C2) and two heterogeneous groups of strains (B and D). For comparison, three strains from each AFLP subcluster and group were subjected to multilocus sequence typing (MLST) analysis. The closest relationship and lowest diversity were found for patterns clustering within AFLP subcluster A1, which corresponded with sequence type (ST) complex 1. Strains within subcluster A1 were mainly invasive cps1 and mrp+ epf+ (or epf*) sly+ cps2+ strains of porcine or human origin. A new finding of this study was the clustering of invasive mrp* cps9 isolates within subcluster A2. MLST analysis suggested that A2 correlates with a single ST complex (ST87). In contrast to A1 and A2, subclusters C1 and C2 contained mainly pneumonia isolates of genotype cps7 or cps2 and epf– sly–. In conclusion, this study demonstrates that AFLP allows identification of clusters of S. suis strains with clinical relevance.

Abbreviations: AFLP, amplified fragment length polymorphism; MLST, multilocus sequence typing; ST, sequence type.




This article has been cited by other articles:


Home page
 CVIHome page
C. G. Baums, C. Kock, A. Beineke, K. Bennecke, R. Goethe, C. Schroder, K.-H. Waldmann, and P. Valentin-Weigand
Streptococcus suis Bacterin and Subunit Vaccine Immunogenicities and Protective Efficacies against Serotypes 2 and 9
Clin. Vaccine Immunol., February 1, 2009; 16(2): 200 - 208.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
D. Takamatsu, M. Osaki, P. Tharavichitkul, S. Takai, and T. Sekizaki
Allelic variation and prevalence of serum opacity factor among the Streptococcus suis population
J. Med. Microbiol., April 1, 2008; 57(4): 488 - 494.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. G. Baums, G. J. Verkuhlen, T. Rehm, L. M. G. Silva, M. Beyerbach, K. Pohlmeyer, and P. Valentin-Weigand
Prevalence of Streptococcus suis Genotypes in Wild Boars of Northwestern Germany
Appl. Envir. Microbiol., February 1, 2007; 73(3): 711 - 717.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.