J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bibiloni, R.
Right arrow Articles by Tannock, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bibiloni, R.
Right arrow Articles by Tannock, G. W.
Agricola
Right arrow Articles by Bibiloni, R.
Right arrow Articles by Tannock, G. W.
J Med Microbiol 55 (2006), 1141-1149; DOI: 10.1099/jmm.0.46498-0
© 2006 Society for General Microbiology
ISSN 1473-5644

The bacteriology of biopsies differs between newly diagnosed, untreated, Crohn's disease and ulcerative colitis patients

Rodrigo Bibiloni1, Marco Mangold2, Karen L. Madsen3, Richard N. Fedorak3 and Gerald W. Tannock1,2

1 ,2 Department of Agricultural, Food and Nutritional Science1 and Department of Microbiology and Immunology2 , University of Otago, PO Box 56, Dunedin, New Zealand

3 Department of Medicine, University of Alberta, Edmonton, Canada

Correspondence
G. W. Tannock
gerald.tannock{at}stonebow.otago.ac.nz

Received 4 January 2006
Accepted 18 April 2006

The bacterial community (microbiota) that inhabits the gut of humans appears to be an important source of antigens that drive the chronic immunological processes characteristic of Crohn's disease (CD) and ulcerative colitis (UC). Most of the members of the microbiota have not yet been cultured, but nucleic-acid-based methods of detection and enumeration can provide information about the community. This investigation used these methods to obtain information about the bacteria associated with mucosal surfaces in the gut of 20 CD and 15 UC patients. Biopsies were collected from inflamed and non-inflamed sites in the intestines of newly diagnosed, untreated patients. Biopsies were also collected from several intestinal sites of 14 healthy subjects. The bacterial collections associated with the biopsies were analysed by generating PCR/denaturing gradient gel electrophoresis (DGGE) profiles, the preparation of 16S rRNA gene clone libraries, and qualitative PCR to detect specific groups of bacteria. The total numbers of bacteria associated with the biopsies were determined by real-time quantitative PCR. DGGE profiles generated from the terminal ileum and various colonic regions were characteristic of each individual but differed between subjects. DGGE profiles and 16S rRNA gene libraries showed that the bacteria associated with inflamed and non-inflamed tissues did not differ. UC patients had more bacteria associated with biopsies than did CD patients (P<0.01). Statistical analysis of the composition of 16S rRNA gene libraries showed that the bacterial collections in UC and CD patients differed (P<0.05). Unclassified members of the phylum Bacteroidetes were more prevalent in CD than in UC patients. Therefore, the types and numbers of bacteria associated with biopsy samples were distinctly different for UC and CD patients. The observations made in this study should permit targeting of specific bacteriological abnormalities in investigations of the pathogenesis of inflammatory bowel diseases and provide targets for medical interventions.

Abbreviations: CD, Crohn's disease; DGGE, denaturing gradient gel electrophoresis; IBD, inflammatory bowel disease; OTU, operational taxonomic unit; UC, ulcerative colitis.




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
S. K. Bhandari, B. Xu, C. M. Nyachoti, D. W. Giesting, and D. O. Krause
Evaluation of alternatives to antibiotics using an Escherichia coli K88+ model of piglet diarrhea: Effects on gut microbial ecology
J Anim Sci, April 1, 2008; 86(4): 836 - 847.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
R. Bibiloni, P. Tandon, F. Vargas-Voracka, R. Barreto-Zuniga, A. Lupian-Sanchez, M. A. Rico-Hinojosa, J. Guban, R. Fedorak, and G. W. Tannock
Differential clustering of bowel biopsy-associated bacterial profiles of specimens collected in Mexico and Canada: what do these profiles represent?
J. Med. Microbiol., January 1, 2008; 57(1): 111 - 117.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
I. Nadal, E. Donant, C. Ribes-Koninckx, M. Calabuig, and Y. Sanz
Imbalance in the composition of the duodenal microbiota of children with coeliac disease
J. Med. Microbiol., December 1, 2007; 56(12): 1669 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
S. Nuding, K. Fellermann, J. Wehkamp, and E. F Stange
Reduced mucosal antimicrobial activity in Crohn's disease of the colon
Gut, September 1, 2007; 56(9): 1240 - 1247.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.