J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nomura, R.
Right arrow Articles by Ooshima, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nomura, R.
Right arrow Articles by Ooshima, T.
Agricola
Right arrow Articles by Nomura, R.
Right arrow Articles by Ooshima, T.
J Med Microbiol 55 (2006), 1135-1140; DOI: 10.1099/jmm.0.46609-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Isolation and characterization of Streptococcus mutans in heart valve and dental plaque specimens from a patient with infective endocarditis

Ryota Nomura1, Kazuhiko Nakano1, Hirotoshi Nemoto1, Kazuyo Fujita1, Satoko Inagaki1, Toshiki Takahashi2, Kazuhiro Taniguchi2, Munehiro Takeda3, Hideo Yoshioka3, Atsuo Amano4 and Takashi Ooshima1

1 ,4 Departments of Pediatric Dentistry1 and Oral Frontier Biology4 , Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan

2 ,3 Departments of Cardiovascular Surgery2 and Dentistry and Oral Surgery3 , Osaka Rosai Hospital, Sakai, Osaka 591-8025, Japan

Correspondence
Kazuhiko Nakano
nakano{at}dent.osaka-u.ac.jp

Received 6 March 2006
Accepted 11 May 2006

Streptococcus mutans, known to be an aetiologic agent of dental caries, also causes infective endocarditis (IE), although a comparison of isolates from the oral cavity and infected heart valve of the same patient has not been reported. In the present study, infected heart valve and dental plaque samples from a patient with IE were analysed. Broad-range PCR with DNA sequencing revealed that 50 clones from the dental plaque isolates were composed of oral streptococci and periodontopathic bacteria, whereas only Streptococcus mutans was detected in 50 clones from the heart valve. Eighteen strains of Streptococcus mutans were isolated from dental plaque and seven from the heart valve, and the biochemical properties of each were in accordance with those of Streptococcus mutans. DNA fingerprinting analysis revealed that all the oral isolates of Streptococcus mutans had similar patterns, which were different from those of the isolates from the infected heart valve. Western blotting using glucosyltransferase (GTF)-specific antiserum showed that the seven strains from the heart valve lacked the three types of intact GTF. In addition, the sucrose-dependent adhesion rates of these isolates were significantly lower than those of the oral isolates (P<0.001). Furthermore, the isolates from the heart valve were less susceptible to erythromycin and kanamycin. These results indicate that the properties of the Streptococcus mutans strains isolated from the infected valve were different from those of typical oral strains, which may be related to the effects of IE.

Abbreviations: AP-PCR, arbitrarily primed PCR; GTF, glucosyltransferase; IE, infective endocarditis; IL-6, interleukin-6; MIC, minimum inhibitory concentration; PAc, protein antigen c; RAPD, random amplified polymorphic DNA.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
H. Nemoto, K. Nakano, R. Nomura, and T. Ooshima
Molecular characterization of Streptococcus mutans strains isolated from the heart valve of an infective endocarditis patient
J. Med. Microbiol., July 1, 2008; 57(7): 891 - 895.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
I. T. Carmona, P. Diz Dios, and C. Scully
Efficacy of Antibiotic Prophylactic Regimens for the Prevention of Bacterial Endocarditis of Oral Origin
Journal of Dental Research, December 1, 2007; 86(12): 1142 - 1159.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
K. Nakano, R. Nomura, H. Nemoto, T. Mukai, H. Yoshioka, Y. Shudo, H. Hata, K. Toda, K. Taniguchi, A. Amano, et al.
Detection of novel serotype k Streptococcus mutans in infective endocarditis patients
J. Med. Microbiol., October 1, 2007; 56(10): 1413 - 1415.
[Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. Nakano, J. Lapirattanakul, R. Nomura, H. Nemoto, S. Alaluusua, L. Gronroos, M. Vaara, S. Hamada, T. Ooshima, and I. Nakagawa
Streptococcus mutans Clonal Variation Revealed by Multilocus Sequence Typing
J. Clin. Microbiol., August 1, 2007; 45(8): 2616 - 2625.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
R. Nomura, M. Hamada, K. Nakano, H. Nemoto, K. Fujimoto, and T. Ooshima
Repeated bacteraemia caused by Streptococcus mutans in a patient with Sjogren's syndrome
J. Med. Microbiol., July 1, 2007; 56(7): 988 - 992.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
K. Nakano, H. Nemoto, R. Nomura, H. Homma, H. Yoshioka, Y. Shudo, H. Hata, K. Toda, K. Taniguchi, A. Amano, et al.
Serotype distribution of Streptococcus mutans a pathogen of dental caries in cardiovascular specimens from Japanese patients
J. Med. Microbiol., April 1, 2007; 56(4): 551 - 556.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.