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J Med Microbiol 55 (2006), 861-863; DOI: 10.1099/jmm.0.46540-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories

Dihadenys Lemus1, Ernesto Montoro1, Miguel Echemendía1, Anandi Martin2, Françoise Portaels2 and Juan Carlos Palomino2

1 Instituto de Medicina Tropical ‘Pedro Kouri’, Autopista Novia del Mediodía Km 61/2, Apartado Postal 601, La Lisa, La Habana, Cuba

2 Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium

Correspondence
Ernesto Montoro
emontoro{at}ipk.sld.cu

Received 24 January 2006
Accepted 26 February 2006

The nitrate reductase assay (NRA) was used as an alternative method for detection of resistance to the first-line antituberculous drugs isoniazid, rifampicin, ethambutol and streptomycin. A total of 320 strains of Mycobacterium tuberculosis were studied and the results compared with the proportion method (PM) on Löwenstein–Jensen medium. The mean time to obtain results was 10 days and the overall agreement between the NRA and PM was 98.8 %. The NRA was easy to perform and represents a useful tool for rapid and accurate determination of drug-resistant M. tuberculosis strains in low-resource countries.

Abbreviations: EMB, ethambutol; INH, isoniazid; MDR, multidrug-resistant; NRA, nitrate reductase assay; PM, proportion method; RMP, rifampicin; STR, streptomycin.




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