J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nateche, F.
Right arrow Articles by Portaels, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nateche, F.
Right arrow Articles by Portaels, F.
Agricola
Right arrow Articles by Nateche, F.
Right arrow Articles by Portaels, F.
J Med Microbiol 55 (2006), 857-860; DOI: 10.1099/jmm.0.46513-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers

Farida Nateche1,2, Anandi Martin3, Saliha Baraka2, Juan Carlos Palomino3, Safia Khaled2 and Françoise Portaels3

1 Faculté de biologie, Université des Sciences et de Technologie, Houari Boumediène, Alger, Algeria

2 Laboratoire Central de l'Esh El Hadi Flici, Alger, Algeria

3 Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium

Correspondence
Farida Nateche
fnateche{at}yahoo.fr

Received 9 January 2006
Accepted 21 February 2006

This study assessed the performance of a rapid, low-cost, colorimetric method, the resazurin microtitre assay (REMA) plate method, for the detection of resistance to isoniazid and rifampicin in 136 clinical isolates of Mycobacterium tuberculosis from two hospitals in Algiers. MICs were determined and the results were compared with those obtained with the conventional proportion method on Löwenstein–Jensen medium. Excellent results were obtained for the REMA plate method, with a sensitivity of 100 % for both isoniazid and rifampicin and a specificity of 98.3 and 99.2 %, respectively. The REMA plate method appears to be a reliable method for the rapid determination of multidrug-resistant tuberculosis and is a good alternative for use in resource-limited countries such as Algeria.

Abbreviations: DST, drug-susceptibility testing; INH, isoniazid; MDRTB, multidrug-resistant tuberculosis; REMA, resazurin microtitre assay; RIF, rifampicin; TB, tuberculosis.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
D. Affolabi, N. Sanoussi, M. Odoun, A. Martin, L. Koukpemedji, J. C. Palomino, L. Kestens, S. Anagonou, and F. Portaels
Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays
J. Med. Microbiol., August 1, 2008; 57(8): 1024 - 1027.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Martin, F. Portaels, and J. C. Palomino
Colorimetric redox-indicator methods for the rapid detection of multidrug resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis
J. Antimicrob. Chemother., February 1, 2007; 59(2): 175 - 183.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.