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J Med Microbiol 55 (2006), 857-860; DOI: 10.1099/jmm.0.46513-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers

Farida Nateche1,2, Anandi Martin3, Saliha Baraka2, Juan Carlos Palomino3, Safia Khaled2 and Françoise Portaels3

1 Faculté de biologie, Université des Sciences et de Technologie, Houari Boumediène, Alger, Algeria

2 Laboratoire Central de l'Esh El Hadi Flici, Alger, Algeria

3 Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium

Correspondence
Farida Nateche
fnateche{at}yahoo.fr

Received 9 January 2006
Accepted 21 February 2006

This study assessed the performance of a rapid, low-cost, colorimetric method, the resazurin microtitre assay (REMA) plate method, for the detection of resistance to isoniazid and rifampicin in 136 clinical isolates of Mycobacterium tuberculosis from two hospitals in Algiers. MICs were determined and the results were compared with those obtained with the conventional proportion method on Löwenstein–Jensen medium. Excellent results were obtained for the REMA plate method, with a sensitivity of 100 % for both isoniazid and rifampicin and a specificity of 98.3 and 99.2 %, respectively. The REMA plate method appears to be a reliable method for the rapid determination of multidrug-resistant tuberculosis and is a good alternative for use in resource-limited countries such as Algeria.

Abbreviations: DST, drug-susceptibility testing; INH, isoniazid; MDRTB, multidrug-resistant tuberculosis; REMA, resazurin microtitre assay; RIF, rifampicin; TB, tuberculosis.




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