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J Med Microbiol 55 (2006), 523-528; DOI: 10.1099/jmm.0.46246-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens

Dana Nováková1, Ivo Sedlácek1, Roman Pantucek2, Vlastimil Stetina1, Pavel Svec1 and Petr Petrás3

1 Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic

2 Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlárská 2, 611 37, Brno, Czech Republic

3 Reference Laboratory for Staphylococci, National Institute of Public Health, Srobárova 48, 100 42, Praha 10, Czech Republic

Correspondence
Dana Nováková
danan{at}sci.muni.cz

Received 15 July 2005
Accepted 12 January 2006

A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII restriction endonucleases and whole-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S. xylosus, S. equorum and S. succinus. The present study confirms what is believed to be the first occurrence of S. equorum in a relevant human clinical material in the Czech Republic and describes what is believed to be the first-ever isolation of S. succinus from human clinical material.

Abbreviations: CCM, Czech Collection of Microorganisms; CoNS, coagulase-negative staphylococci.

The GenBank/EMBL/DDBJ accession numbers for the partial 16S rDNA sequences of Staphylococcus succinus strains NRL 99/698, CCM 7312, CCM 7313, CCM 7314 and NRL 02/262 are DQ006829–DQ006833, respectively, and those for Staphylococcus equorum strains CCM 7301, NRL 00/738, CCM 7302, NRL 02/216, NRL 99/524 and NRL 99/760 are DQ006834–DQ006839, respectively.






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