J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Watanabe, I.
Right arrow Articles by Mitsuyama, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watanabe, I.
Right arrow Articles by Mitsuyama, M.
Agricola
Right arrow Articles by Watanabe, I.
Right arrow Articles by Mitsuyama, M.
J Med Microbiol 55 (2006), 505-510; DOI: 10.1099/jmm.0.46333-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Dependence of the lethal effect of pore-forming haemolysins of Gram-positive bacteria on cytolytic activity

Isao Watanabe1, Takamasa Nomura1, Takanari Tominaga1, Kazuhiro Yamamoto1, Chikara Kohda2, Ikuo Kawamura1 and Masao Mitsuyama1

1 Department of Microbiology, Kyoto University Graduate School of Medicine, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan

2 Department of Bacteriology, Showa University School of Medicine, Tokyo 142-8555, Japan

Correspondence
Masao Mitsuyama
mituyama{at}mb.med.kyoto-u.ac.jp

Received 16 September 2005
Accepted 12 January 2006

Among bacterial haemolysins, cholesterol-dependent cytolysins (CDCs) produced by various Gram-positive bacteria are known to exhibit a lethal activity in mice. In this study, recombinant CDCs of streptolysin O, pneumolysin, ivanolysin O, listeriolysin O and several listeriolysin O mutants were constructed and the relationship between cytolytic activity and the lethal activity of each recombinant protein in mice was examined. Specific activity for cytolysis was determined by a quantitative haemolytic assay. Each protein was injected intravenously into mice and the lethal activity was evaluated by measuring the time until death of the mice. The four full-length CDC proteins exhibited lethal activity and their activities were highly proportional to their cytolytic activities. Inhibition of haemolytic activity resulted in the loss of lethal activity and non-haemolytic mutants of listeriolysin O did not exhibit any lethal activity. These data clearly indicate that the lethal effect of CDC proteins is dependent on the cytolytic activity.

Abbreviations: CDC, cholesterol-dependent cytolysin; HU, haemolytic unit; ILO, ivanolysin O; LLO, listeriolysin O; PLY, pneumolysin; SLO, streptolysin O.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
J. Chu, L. M. Thomas, S. C. Watkins, L. Franchi, G. Nunez, and R. D. Salter
Cholesterol-dependent cytolysins induce rapid release of mature IL-1{beta} from murine macrophages in a NLRP3 inflammasome and cathepsin B-dependent manner
J. Leukoc. Biol., November 1, 2009; 86(5): 1227 - 1238.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Hara, K. Tsuchiya, T. Nomura, I. Kawamura, S. Shoma, and M. Mitsuyama
Dependency of Caspase-1 Activation Induced in Macrophages by Listeria monocytogenes on Cytolysin, Listeriolysin O, after Evasion from Phagosome into the Cytoplasm
J. Immunol., June 15, 2008; 180(12): 7859 - 7868.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H. Hara, I. Kawamura, T. Nomura, T. Tominaga, K. Tsuchiya, and M. Mitsuyama
Cytolysin-Dependent Escape of the Bacterium from the Phagosome Is Required but Not Sufficient for Induction of the Th1 Immune Response against Listeria monocytogenes Infection: Distinct Role of Listeriolysin O Determined by Cytolysin Gene Replacement
Infect. Immun., August 1, 2007; 75(8): 3791 - 3801.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
T. Nomura, I. Kawamura, C. Kohda, H. Baba, Y. Ito, T. Kimoto, I. Watanabe, and M. Mitsuyama
Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria
Microbiology, July 1, 2007; 153(7): 2250 - 2258.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.