J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iqbal, J.
Right arrow Articles by Sher, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iqbal, J.
Right arrow Articles by Sher, A.
Agricola
Right arrow Articles by Iqbal, J.
Right arrow Articles by Sher, A.
J Med Microbiol 55 (2006), 401-405; DOI: 10.1099/jmm.0.46376-0
© 2006 Society for General Microbiology
ISSN 0022-2615

Determination of the prevalence of lymphatic filariasis among migrant workers in Kuwait by detecting circulating filarial antigen

Jamshaid Iqbal1 and Ali Sher2

1 Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, Kuwait

2 Malaria Laboratory, Ports and Borders Center, Ministry of Health, Kuwait

Correspondence
Jamshaid Iqbal
iqbal{at}hsc.edu.kw

Received 16 October 2005
Accepted 7 December 2005

The main objective of this study was to determine the prevalence of filarial infection among migrant workers in Kuwait. The study was conducted from April 2000 to November 2003. A total of 1050 migrant workers (>90 % from the Indian subcontinent) from filarial endemic countries and 260 individuals residing in Kuwait as controls (50 healthy Kuwaiti blood donors, 50 microfilaria-negative individuals from endemic areas and 160 patients with other parasitic infections) were screened for filarial infection. All specimens were tested for microfilaraemia by microscopy of nucleopore-filtered blood (NFB) and detection of circulating filarial antigen (CFA) by an immunochromatographic test (ICT) and the TropBio assay. The overall prevalence of filarial antigenaemia was 18·3 % (192 individuals) using the ICT and 20·3 % (213 individuals) using the TropBio assay. Thirty-two cases (3 %) of Wuchereria bancrofti were detected by microscopy and the mean microfilaria count in these cases was 816 microfilariae ml–1. CFA was detected only in two of the 260 control subjects. Statistical analysis to calculate the sensitivity, specificity and prevalence of infection was carried out using maximum-likelihood statistical methods. The overall sensitivity and specificity of the ICT and TropBio assay to detect CFA were comparable. Compared with NFB microscopy, the sensitivity of the ICT was 93·8 % and specificity ranged from 84 to 100 %. The sensitivity and specificity of the TropBio assay were 90·1 and 100 %, respectively. However, the ICT failed to detect CFA in two cases with a microfilarial load of <20 microfilariae ml–1. In conclusion, the prevalence of filarial infection among the migrant workers in Kuwait was 18·3 % as determined by the ICT.

Abbreviations: AU, antigen units; CFA, circulating filarial antigen; ICT, immunochromatographic test; NFB, nucleopore-filtered blood; mf, microfilariae; ML, maximum likelihood.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.