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J Med Microbiol 55 (2006), 375-378; DOI: 10.1099/jmm.0.46217-0
© 2006 Society for General Microbiology
ISSN 0022-2615

DNA vaccines expressing pneumococcal surface protein A (PspA) elicit protection levels comparable to recombinant protein

Daniela M. Ferreira, Eliane N. Miyaji, Maria Leonor S. Oliveira, Michelle Darrieux, Ana Paula M. Arêas, Paulo L. Ho and Luciana C. C. Leite

Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, SP, Brazil

Correspondence
Eliane N. Miyaji
enmiyaji{at}butantan.gov.br

Received 29 June 2005
Accepted 16 November 2005

Pneumococcal surface protein A (PspA) is a promising candidate for the development of cost-effective vaccines against Streptococcus pneumoniae. In the present study, BALB/c mice were immunized with DNA vaccine vectors expressing the N-terminal region of PspA. Animals immunized with a vector expressing secreted PspA developed higher levels of antibody than mice immunized with the vector expressing the antigen in the cytosol. However, both immunogens elicited similar levels of protection against intraperitoneal challenge. Furthermore, immunization with exactly the same fragment in the form of a recombinant protein, with aluminium hydroxide as an adjuvant, elicited even higher antibody levels, but this increased humoral response did not correlate with enhanced protection. These results show that DNA vaccines expressing PspA are able to elicit protection levels comparable to recombinant protein, even though total anti-PspA IgG response is considerably lower.

Abbreviations: BHK, baby hamster kidney; rPspA, recombinant PspA.




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D. M. Ferreira, M. Darrieux, M. L. S. Oliveira, L. C. C. Leite, and E. N. Miyaji
Optimized Immune Response Elicited by a DNA Vaccine Expressing Pneumococcal Surface Protein A Is Characterized by a Balanced Immunoglobulin G1 (IgG1)/IgG2a Ratio and Proinflammatory Cytokine Production
Clin. Vaccine Immunol., March 1, 2008; 15(3): 499 - 505.
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