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J Med Microbiol 55 (2006), 365-373; DOI: 10.1099/jmm.0.46257-0
© 2006 Society for General Microbiology
ISSN 0022-2615

Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles

Sara M. Soto1,2, Irene Rodríguez1, M. Rosario Rodicio1, Jordi Vila2 and M. Carmen Mendoza1

1 Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, C/Julián Clavería 6, 33006 Oviedo, Spain

2 Servei de Microbiología, Centre de Diagnòstic Biologic, Hospital Clinic-IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain

Correspondence
M. Carmen Mendoza
cmendoza{at}uniovi.es

Received 22 July 2005
Accepted 21 December 2005

A total of 80 strains of Salmonella enterica serovar Enteritidis, causing gastroenteritis (G) or bacteraemia (B), and three control strains (C), were subjected to: (i) detection of 14 chromosomally and 1 plasmid-located virulence genes by PCR, (ii) detection of DNA polymorphisms by XbaI and BlnI PFGE, and cluster analysis, (iii) mapping of the 15 screened sequences on macrorestriction profiles and (iv) comparison of the screening and mapping results with data available for other Salmonella strains. Identical virulence genotypes and very similar macrorestriction profiles were shown by most S. Enteritidis strains. However, a number of B strains belonged to genomic types with polymorphisms affecting fragments carrying (SPI2-slyA), (SPI2-slyA-phoP/Q-agfA), (SPI4 and/or stn) and spvC. The information obtained provides the basis for further studies on the genetic background of virulence and the molecular epidemiology of S. Enteritidis.

Abbreviations: SPI, Salmonella pathogenicity island.






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