J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
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J Med Microbiol 55 (2006), 201-206; DOI: 10.1099/jmm.0.46110-0
© 2006 Society for General Microbiology
ISSN 0022-2615

ent-Epiafzelechin-(4{alpha}->8)-epiafzelechin extracted from Cassia javanica inhibits herpes simplex virus type 2 replication

Hua-Yew Cheng1, Chien-Min Yang2, Ta-Chen Lin3, Den-En Shieh4 and Chun-Ching Lin2

1 Department of Cosmetic Applications & Management, Tung Fang Institute of Technology, 829 Kaohsiung County, Taiwan

2 Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, 807 Kaohsiung, Taiwan

3 General Education Center, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan

4 Department of Food Science and Technology, Tajen Institute of Technology, 907 Ping-Tung, Taiwan

Correspondence
Chun-Ching Lin
aalin{at}ms24.hinet.net

Received 7 April 2005
Accepted 11 October 2005

Herpes simplex virus (HSV) is a ubiquitous organism that causes infections in human populations throughout the world. It causes a variety of diseases ranging in severity from mild to life-threatening. In this study, ent-epiafzelechin-(4{alpha}->8)-epiafzelechin (EEE) extracted from the fresh leaves of Cassia javanica L. agnes de Wit (Leguminosae) was investigated for its in vitro anti-HSV-2 activity using XTT and plaque reduction assays. Results showed that EEE inhibited HSV-2 replication in a dose-dependent manner. The IC50 value was 83·8±10·9 and 166·8±12·9 µM for XTT and plaque reduction assays, respectively. EEE did not affect the viability and the proliferation of cells at antiviral concentrations. Mechanistic studies demonstrated that EEE prevented HSV-2 from penetrating the cell and also interfered with HSV-2 replication at the late stage of its life cycle. It also disturbed virus attachment but the inhibitory effect was minor. In summary, the conclusion of this study was that EEE exhibits various modes of action in suppressing HSV-2 multiplication.

Abbreviations: ACV, acyclovir; 33'DOGPB, 3,3'-di-O-galloyl prodelphinidin B-2; EEE, ent-epiafzelechin-(4{alpha}->8)-epiafzelechin; 3OGPB, 3'-O-galloyl prodelphinidin B-2; SI, selectivity index; XTT, sodium 3'-[1-(phenylamino-carbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid.






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