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J Med Microbiol 55 (2006), 165-170; DOI: 10.1099/jmm.0.46287-0
© 2006 Society for General Microbiology
ISSN 0022-2615

Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage

Je Hee Lee1,2, Kyung Ho Han1, Seon Young Choi1,2, Marcelino E. S. Lucas3, C. Mondlane4, M. Ansaruzzaman5, G. Balakrish Nair5, David A. Sack5, Lorenz von Seidlein1, John D. Clemens1, Manki Song1, Jongsik Chun1,2, The Mozambique Cholera Vaccine Demonstration Project Coordination Group and Dong Wook Kim1

1 International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea

2 School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea

3 Ministry of Health, Maputo, Mozambique

4 Centro De Higiene Ambiental E Exames Medicos, Rua Correia De Brito N° 1815 Sofala – Beira, Mozambique

5 International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

Correspondence
Dong Wook Kim
dwkim{at}ivi.int

Received 10 August 2005
Accepted 26 September 2005

Vibrio cholerae O1 isolates belonging to the Ogawa serotype, El Tor biotype, harbouring the classical CTX prophage were first isolated in Mozambique in 2004. Multilocus sequence typing (MLST) analysis using nine genetic loci showed that the Mozambique isolates have the same sequence type (ST) as O1 El Tor N16961, a representative of the current seventh cholera pandemic. Analysis of the CTX prophage in the Mozambique isolates indicated that there is one type of rstR in these isolates: the classical CTX prophage. It was also found that the ctxB-rstR-rstA-rstB-phs-cep fragment was PCR-amplified from these isolates, which indicates the presence of a tandem repeat of the classical CTX prophage in the genome of the Mozambique isolates. The possible origin of these isolates and the presence of the tandem repeat of the classical prophage in them implicate the presence of the classical CTX phage.

Abbreviations: IVI, International Vaccine Institute; MLST, multilocus sequence typing; ST, sequence type.

The GenBank/EMBL/DDBJ accession numbers for the sequences of the new allele types are DQ012291–DQ012295.




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