J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Bacteriophage sequence
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pitcher, D.
Right arrow Articles by Harrison, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pitcher, D.
Right arrow Articles by Harrison, T. G.
Agricola
Right arrow Articles by Pitcher, D.
Right arrow Articles by Harrison, T. G.
J Med Microbiol 55 (2006), 149-155; DOI: 10.1099/jmm.0.46281-0
© 2006 Society for General Microbiology
ISSN 0022-2615

Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control

David Pitcher, Victoria J. Chalker, Carmen Sheppard, Robert C. George and Timothy G. Harrison

Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Correspondence
Victoria J. Chalker
vicki.chalker{at}hpa.org.uk

Received 4 August 2005
Accepted 16 September 2005

Real-time PCR was employed to detect a region of the P1 cytadhesin gene of Mycoplasma pneumoniae in clinical samples. An internal processing control was included that could be co-amplified simultaneously in the same reaction tube. The assay could reproducibly detect 1x103 M. pneumoniae organisms ml–1 in clinical samples. There was no amplification of DNA or signal production from 15 other species of human mycoplasmas and 19 other bacterial species. Using a panel of 175 respiratory samples taken from patients with pneumonia of proven aetiology, the sensitivity was found to be 60 % and the specificity of the assay 96·7 % when compared with serology. This assay is suitable for same-day diagnosis of M. pneumoniae infection and batch processing of respiratory samples for clinical screening.

Abbreviations: Cp, crossing point; IPC, internal processing control.

The sequence of the internal processing control bacteriophage lambda fragment is available as supplementary material in JMM Online.




This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
A. Touati, A. Benard, A. B. Hassen, C. M. Bebear, and S. Pereyre
Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens
J. Clin. Microbiol., July 1, 2009; 47(7): 2269 - 2271.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. Dumke and E. Jacobs
Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae
J. Clin. Microbiol., February 1, 2009; 47(2): 441 - 444.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. M. Winchell, K. A. Thurman, S. L. Mitchell, W. L. Thacker, and B. S. Fields
Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation
J. Clin. Microbiol., September 1, 2008; 46(9): 3116 - 3118.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. Dumke, N. Schurwanz, M. Lenz, M. Schuppler, C. Luck, and E. Jacobs
Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real-Time PCR Approach
J. Clin. Microbiol., August 1, 2007; 45(8): 2726 - 2730.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.