J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rigden, R. C.
Right arrow Articles by Murray, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rigden, R. C.
Right arrow Articles by Murray, A.
Agricola
Right arrow Articles by Rigden, R. C.
Right arrow Articles by Murray, A.
J Med Microbiol 55 (2006), 1735-1740; DOI: 10.1099/jmm.0.46785-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Humoral and cellular immune responses in sheep immunized with a 22 kilodalton exported protein of Mycobacterium avium subspecies paratuberculosis

Rachael C. Rigden1,{dagger}, Dakshina M. Jandhyala1,{dagger}, Chris Dupont1,{ddagger}, Dianna Crosbie-Caird1, Nicolas Lopez-Villalobos1, Norihiro Maeda2, Brigitte Gicquel2 and Alan Murray1,2

1 Institute of Veterinary, Animal and Biomedical Science, Massey University, Private Bag 11 222, Palmerston North, New Zealand

2 Unité de Génétique Mycobactérienne, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France

Correspondence
Rachael C. Rigden
r.c.rigden{at}massey.ac.nz

Received 15 June 2006
Accepted 21 August 2006

An immunogenic 22 kilodalton exported Mycobacterium avium subspecies paratuberculosis (MAP) lipoprotein (P22) was previously identified, and found to belong to the LppX/LprAFG family of mycobacterial lipoproteins. N-terminal polyhistidine-tagged P22 was produced and purified from Escherichia coli. Antibody recognition of P22, and interferon-gamma (IFN-{gamma}) responses in vitro using blood from a sheep vaccinated with Neoparasec, confirmed its immunogenicity. To evaluate the immunogenicity of P22 in vivo, five sheep were immunized with a single dose containing 0.8 mg recombinant P22 protein in adjuvant. Blood was collected at 4, 13 and 29 weeks post-immunization (p.i.) and tested for anti-P22 antibodies and P22-specific IFN-{gamma} production. P22-specific antibodies were detected by Western blot analysis in all five Neoparasec-immunized sheep at the three time points. Three out of five P22-immunized sheep produced P22-specific antibodies for up to 13 weeks p.i., and two gave a response at 29 weeks p.i. Recombinant P22 was able to stimulate significant IFN-{gamma} production in blood of P22-immunized sheep at 13 and 29 weeks p.i. Recombinant P22 also elicited an IFN-{gamma} response in blood of sheep immunized with Neoparasec.

Abbreviations: Ab, antibody; Ag, antigen; dH2O, deionized water; HRP, horseradish peroxidase; IFN-{gamma}, interferon-gamma; JD, Johne's disease; MAP, Mycobacterium avium subspecies paratuberculosis; MTB, Mycobacterium tuberculosis; P22, 22kDa exported MAP protein; p.i., post-immunization; PPDA, avian purified protein derivative; RT, room temperature.

{dagger}These authors contributed equally to this work.

{ddagger}Present address: Department of Biology, University of Waterloo, 200 University Avenue W., Waterloo, ON N2L 3G1, Canada.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
J. P. Bannantine, M. L. Paustian, W. R. Waters, J. R. Stabel, M. V. Palmer, L. Li, and V. Kapur
Profiling Bovine Antibody Responses to Mycobacterium avium subsp. paratuberculosis Infection by Using Protein Arrays
Infect. Immun., February 1, 2008; 76(2): 739 - 749.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2006 Society for General Microbiology.