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J Med Microbiol 55 (2006), 1725-1734; DOI: 10.1099/jmm.0.46726-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Yersinia enterocolitica isolates of differing biotypes from humans and animals are adherent, invasive and persist in macrophages, but differ in cytokine secretion profiles in vitro

Alan McNally1,{dagger}, Tracey Dalton2, Roberto M. La Ragione1, Kenneth Stapleton1, Georgina Manning1,{dagger} and Diane G. Newell1

1 Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey KT15 3NB, UK

2 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E, UK

Correspondence
Diane G. Newell
d.newell{at}vla.defra.gsi.gov.uk

Received 16 May 2006
Accepted 12 August 2006

Previous epidemiological studies have demonstrated a potential link between the serotypes of Yersinia enterocolitica recovered from cattle, sheep and pigs and those isolated from human disease cases. Further studies utilizing amplified fragment length polymorphisms have shown a relationship at the genetic level between strains of biotypes 3 and 4 from humans and livestock, and also suggested that some biotype 1A isolates, classically defined as non-pathogenic, are closely related to biotype 3 and 4 isolates. This study sought to understand further the pathogenic potential of Y. enterocolitica isolates from livestock in Great Britain. A range of surrogate in vitro models, such as invasion of epithelial tissue cultures, survival in cultured macrophages and cytokine secretion response, was employed to assess the pathogenicity of 88 strains. The results suggested that all isolates examined were capable of adhering to and invading epithelial cells and of surviving within macrophages. However, the inflammatory response of the infected macrophages differed with the infecting Y. enterocolitica subtype, with the response to pathogenic biotype 3 and 4 isolates different to that observed with biotype 1A isolates, and with the biotype 3 O : 5,27 isolates recovered exclusively from animals. Infections of porcine tissue also suggested the possibility of host-tissue tropism within Y. enterocolitica subtypes.

Abbreviations: AFLP, amplified fragment length polymorphism; GFP, green fluorescent protein; IL, interleukin; IVOC, in vitro organ culture; TNF, tumour necrosis factor.

{dagger}Present address: School of Biomedical and Natural Science, Nottingham Trent University, Clifton Lane, Nottingham, UK.




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A. McNally, R. M. La Ragione, A. Best, G. Manning, and D. G. Newell
An aflagellate mutant Yersinia enterocolitica biotype 1A strain displays altered invasion of epithelial cells, persistence in macrophages, and cytokine secretion profiles in vitro
Microbiology, May 1, 2007; 153(5): 1339 - 1349.
[Abstract] [Full Text] [PDF]


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