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J Med Microbiol 55 (2006), 1711-1715; DOI: 10.1099/jmm.0.46593-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Rapid identification of clinically relevant Nocardia species using real-time PCR with SYBR Green and melting-curve analysis

Mubarak Alfaresi and Abida Elkosh

Department of Pathology and Laboratory Medicine – Microbiology Division, Zayed Military Hospital, Abu Dhabi, United Arab Emirates

Correspondence
Mubarak Alfaresi
uaenow{at}emirates.net.ae

Received 26 February 2006
Accepted 21 August 2006

The objective of this study was to develop and evaluate a rapid new method of identifying clinically relevant Nocardia species. DNA extracted from different Nocardia strains was used in a real-time PCR assay with SYBR Green and melting-curve analysis to identify Nocardia species. Ten control strains and four bacterial strains of closely related genera were employed, and samples from 28 patients were used. All Nocardia strains were identified correctly, and there was no cross-reaction with strains from genera closely related to Nocardia. The sensitivity and specificity of the method were 90 and 100 %, respectively. This method can be used to rapidly detect Nocardia species in culture, without cross-reaction with other closely related genera.




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