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J Med Microbiol 55 (2006), 1707-1710; DOI: 10.1099/jmm.0.46754-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Use of sequence-based typing for investigation of a case of nosocomial legionellosis

Sallene Wong1, Kanti Pabbaraju1, Veronica F. Burk1, George C. Broukhanski2, Julie Fox1,3, Tom Louie4, Manuel W. Mah4, Kathryn Bernard5 and Peter A. G. Tilley1,3

1 Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada

2 Ontario Ministry of Health and Long-Term Care, Toronto, Ontario, Canada

3 Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada

4 Infection Prevention and Control, Calgary Health Region, Calgary, Alberta, Canada

5 National Microbiology Laboratory, Winnipeg, Manitoba, Canada

Correspondence
Peter A. G. Tilley
p.tilley{at}provlab.ab.ca

Received 3 June 2006
Accepted 11 August 2006

A fatal case of nosocomial legionellosis in a low prevalence region (Calgary, Alberta, Canada) prompted investigation into the source of infection. Hospital water systems contaminated with Legionella pneumophila have been shown to pose a risk to compromised patients. Typing of an L. pneumophila serogroup 1 strain isolated from the patient using sequence-based typing (SBT) and amplified fragment length polymorphism (AFLP) analysis linked it to a persistent and widespread strain isolated from the hospital water system establishing a nosocomial mode of acquisition. Different SBT and AFLP patterns were determined for non-epidemiologically linked cases and isolates from different hospitals.

Abbreviations: AFLP, amplified fragment length polymorphism; ER, emergency room; EWGLI, European Working Group for Legionella Infections; ICU, intensive care unit; SBT, sequence-based typing.

The GenBank accession numbers for the novel alleles of mompS and proA are DQ309456 and DQ309457, respectively.




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