J Med Microbiol 55 (2006), 1661-1665; DOI: 10.1099/jmm.0.46792-0
© 2006 Society for General Microbiology
ISSN 1473-5644
Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356
Kyung Ho Han1,2,
,
Seon Young Choi1,3,,
Je Hee Lee1,3,
Hyejon Lee1,
Eun Hee Shin1,
Magdarina D. Agtini4,
Lorenz von Seidlein1,
R. Leon Ochiai1,
John D. Clemens1,
John Wain5,
Ji-Sook Hahn2,6,
Bok Kwon Lee7,
Manki Song1,
Jongsik Chun1,3 and
Dong Wook Kim1
1 International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea
2 ,3 ,6 Interdisciplinary Program for Biochemical Engineering and Biotechnology2 , School of Biological Sciences3 and School of Chemical and Biological Engineering6 , Seoul National University, Seoul, 151-742, Republic of Korea
4 National Institute of Health Research and Development, Ministry of Health, Jakarta, 10560, Indonesia
5 The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK
7 Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health Korea, Republic of Korea
Correspondence
Dong Wook Kim
dwkim{at}ivi.int
Received 19 June 2006
Accepted 21 August 2006
Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT– (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT+ (S. Paratyphi B dT+; formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT+ S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d-tartrate, reported elsewhere, was validated. The d-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT– reference strain was changed to the dT+ phenotype by complementing ORF STM 3356 in trans. The results show that the dT+ phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method.
Abbreviations: MDR, multidrug-resistant; MLST, multilocus sequence typing; SGI1, Salmonella genomic island 1.
These two authors contributed equally to this work.
Copyright © 2006 Society for General Microbiology.
