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J Med Microbiol 55 (2006), 1641-1644; DOI: 10.1099/jmm.0.46845-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis

Lucas Smith1, Barbara Rose1, Pholawat Tingpej1, Hua Zhu2, Tim Conibear3, Jim Manos1, Peter Bye4,5, Mark Elkins4, Mark Willcox2,3, Scott Bell6,7, Claire Wainwright8,9 and Colin Harbour1

1 ,5 Department of Infectious Diseases and Immunology, Blackburn Building DO61 and Department of Medicine5 , University of Sydney, Sydney, Australia

2 Institute for Eye Research, Sydney, Australia

3 School of Optometry and Vision Science, The University of New South Wales, Sydney, Australia

4 Department of Respiratory Medicine, Royal Prince Alfred Hospital, Sydney, Australia

6 Adult Cystic Fibrosis Centre, The Prince Charles Hospital, Brisbane, Australia

7 ,9 Department of Medicine7 and Department of Paediatrics and Child Health9 , University of Queensland, Brisbane, Australia

8 Department of Respiratory Medicine, Royal Children's Hospital, Brisbane, Australia

Correspondence
Colin Harbour
charbour{at}infdis.usyd.edu.au

Received 19 July 2006
Accepted 11 August 2006

Protease IV is important in the pathogenesis of Pseudomonas aeruginosa-induced microbial keratitis, but little is known of its role in cystic fibrosis (CF) lung infection. In this study protease IV production was examined in 43 P. aeruginosa isolates (24 non-clonal and 19 clonal) from the lungs of chronically infected adult patients attending the Royal Prince Alfred Hospital CF Clinic, Sydney, Australia. Overall, 32/43 (74 %) isolates were positive for protease IV protein by Western blotting and 22/43 (51 %) had evidence of active protease IV on gelatin zymography. Clonal strains were 1.6 times more likely than non-clonal strains to produce protease IV [18/19 (95 %) versus 14/24 (58 %), RR=1.6, CI 1.1–2.3, P=0.007] and 3 times more likely to secrete the protein [16/19 (84 %) versus 6/24 (25 %), RR=3.4, CI 1.6–6.9, P<0.001]. Nine of the ten strains negative by both Western blotting and zymography were non-clonal, and all but one of these was positive for the protease IV gene. There was a marked strain-to-strain variation in the amount of protease IV produced. Secretion of protease IV by clonal strains may enhance their infectivity and ability to adapt to the changing CF lung environment. Overall the findings suggest that protease IV plays an important role in the pathogenesis of P. aeruginosa infection in the CF lung.

Abbreviations: CF, cystic fibrosis; CI, confidence interval; RR, relative risk.




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