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J Med Microbiol 55 (2006), 1563-1569; DOI: 10.1099/jmm.0.46689-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Genetic characteristics of Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes

Ashrafus Safa1, N. A. Bhuyian1, Suraia Nusrin1, M. Ansaruzzaman1, Munirul Alam1, T. Hamabata2, Yoshifumi Takeda3, David A. Sack1 and G. Balakrish Nair1

1 Laboratory Sciences Division, Enteric Microbiology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Centre for Health and Population Research, GPO Box 128, Dhaka 1000, Bangladesh

2 International Medical Research Center of Japan, Toyama, Tokyo, Japan

3 Cine-Science Laboratory, Tokiwadai, Tokyo, Japan

Correspondence
G. Balakrish Nair
gbnair{at}icddrb.org

Received 20 April 2006
Accepted 18 July 2006

The Matlab variants of Vibrio cholerae O1, defined as hybrids between the classical and El Tor biotypes, were first isolated from hospitalized patients with acute secretory diarrhoea in Matlab, a rural area of Bangladesh. These variants could not be categorized as classical or El Tor biotypes by phenotypic and genotypic tests, and had representative traits of both the biotypes. A number of virulence-associated genes and/or gene clusters were screened by PCR and DNA sequencing. El Tor-specific gene clusters, Vibrio seventh-pandemic islands (VSP)-I and -II and repeat toxin (RTX) were present in the genome of these variants, indicating their El Tor lineage, whereas the nucleotide-sequence-derived CtxB amino acid sequence of these strains grouped them under the classical biotype. Matlab variants possessed all the necessary genes to initiate pandemics. The genetic relatedness of Matlab variants to the V. cholerae strains recently isolated in Mozambique is another important observation of this study, which underscores the epidemiological significance of Matlab variants.

Abbreviations: CCA, chicken red cell agglutination; CT, cholera toxin; MSHA, mannose-sensitive haemagglutinin; RTX, repeat toxin; TLC, toxin-linked cryptic; VPI, Vibrio pathogenicity island.

The GenBank/EMBL/DDBJ accession numbers for the ctxB gene sequences of Vibrio cholerae O1 and O139 are DQ523199–DQ523219.




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