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J Med Microbiol 55 (2006), 1499-1504; DOI: 10.1099/jmm.0.46621-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Antigenicity of borrelial protein BBK32 fragments in early Lyme borreliosis

Pekka Lahdenne1, Heikki Sarvas2,3, Riikka Kajanus2, Miia Eholuoto2, Heidi Sillanpää2 and Ilkka Seppälä2,3

1 Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, FIN-00290 Helsinki, Finland

2 Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland

3 HUSLAB Laboratory Diagnostics, Helsinki University Central Hospital, Haartmaninkatu 3, FIN-00290 Helsinki, Finland

Correspondence
Pekka Lahdenne
pekka.lahdenne{at}hus.fi

Received 10 March 2006
Accepted 8 August 2006

Recombinantly produced borrelial BBK32 protein fragments originating from Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii were evaluated as antigens in the serology of Lyme borreliosis (LB). In ELISA, a mid-portion hydrophilic fragment reacted with LB patient sera. Of the 23 patients with culture- or PCR-positive erythema migrans (EM), 43 % at diagnosis and 52 % at convalescence were positive for at least one Borrelia species-specific variant BBK32 fragment antigen. In parallel ELISAs with BBK32 whole proteins from the three borrelial subspecies as antigens, 17 % at diagnosis and 26 % at convalescence were positive. These results suggest that BBK32 protein fragments may improve the early IgG serology of LB compared to the BBK32 whole protein.

Abbreviations: ACA, acrodermatitis chronica atrophicans; ASO, anti-streptolysin; CDC, Centers for Disease Control; EM, erythema migrans; LA, Lyme arthritis; LB, Lyme borreliosis; NB, neuroborreliosis; RF, rheumatoid factor.




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M. He, B. K. Boardman, D. Yan, and X. F. Yang
Regulation of Expression of the Fibronectin-Binding Protein BBK32 in Borrelia burgdorferi
J. Bacteriol., November 15, 2007; 189(22): 8377 - 8380.
[Abstract] [Full Text] [PDF]


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