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J Med Microbiol 55 (2006), 1477-1485; DOI: 10.1099/jmm.0.46660-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model

Takako Osaki1, Tomoko Hanawa1, Taki Manzoku1, Minoru Fukuda2, Hayato Kawakami3, Hidekazu Suzuki4, Hiroyuki Yamaguchi5, Xu Yan6, Haruhiko Taguchi1, Satoshi Kurata1 and Shigeru Kamiya1

1 ,2 ,3 Department of Infectious Diseases1 , Laboratory for Electron Microscopy2 and Second Department of Anatomy3 , Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan

4 Department of Internal Medicine, Keio University School of Medicine Shinjuku-ku, Tokyo, Japan

5 Department of Basic Laboratory Sciences, School of Allied Health Sciences, Osaka University, Yamadaoka, Suita, Osaka, Japan

6 Department of Microbiology, Kunming Medical School, Kunming, Republic of China

Correspondence
Takako Osaki
osaki{at}kyorin-u.ac.jp

Received 4 April 2006
Accepted 18 July 2006

Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (AI-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0.3 % agar medium, motility of H. pylori HPKY08 (luxS : : cat) was significantly lower than that of wild-type H. pylori TK1402. The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/AI-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS– mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.

Abbreviations: AI-2, type 2 autoinducer; qRT-PCR, semi-quantitative real-time reverse transcription PCR.




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