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J Med Microbiol 55 (2006), 1389-1393; DOI: 10.1099/jmm.0.46706-0
© 2006 Society for General Microbiology
ISSN 1473-5644

Construction of a eukaryotic expression system for granulysin and its protective effect in mice infected with Mycobacterium tuberculosis

Binbo Liu1, Shengwu Liu2, Xueju Qu2 and Junyan Liu2

Department of Microbiology1 and Department of Immunology2 , School of Medicine, Wuhan University, Wuhan 430071, People's Republic of China

Correspondence
Binbo Liu
lbbbbl{at}163.com

Received 3 May 2006
Accepted 27 June 2006

A full-length cDNA of granulysin was inserted into the pcDNA3.1(–) vector to construct a eukaryotic expression plasmid for granulysin. The recombinant plasmids were injected intramuscularly into mice infected with Mycobacterium tuberculosis to evaluate the protective effect of granulysin. Granulysin significantly decreased the weight index (WI) of the spleen, reduced the numbers of viable bacteria in lung and spleen, and reduced the lesions of lung tissue in granulysin-rDNA-immunized mice compared with those of control group mice. In vitro, the serum of the recombinant-plasmid-immunized mice inhibited the viability of M. tuberculosis by the physical disruption of cell membranes. Therefore, granulysin has a therapeutic effect against M. tuberculosis.

Abbreviations: CTL, cytotoxic T lymphocyte; H&E, haematoxylin and eosin; NK cell, natural killer cell; PBMC, peripheral blood mononuclear cell; TB, tuberculosis; WI, weight index.






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