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J Med Microbiol 55 (2006), 127-131; DOI: 10.1099/jmm.0.46218-0
© 2006 Society for General Microbiology
ISSN 0022-2615

Case Report

Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods

Philip Suffys1, Adalgiza da Silva Rocha1, Adeilton Brandão2, Bart Vanderborght3, Wouter Mijs4, Geert Jannes4, Fernanda C. Q. Mello3, Heloisa da Silveira Paro Pedro5, Leila de Souza Fonseca6, Rosângela Siqueira de Oliveira7, Sylvia Cardoso Leão7 and Maria Helena Féres Saad1

1 ,2 Department of Mycobacterioses1 and Department of Tropical Medicine2 , Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil

3 ,6 University Hospital Clementino Fraga Filho3 and Microbiology Institute6 , Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

4 Innogenetics N. V., Technologiepark 6, B-9052 Ghent, Belgium

5 Adolfo Lutz Institute, São Paulo, Brazil

7 Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, São Paulo, Brazil

Correspondence
Philip Suffys
psuffys{at}ioc.fiocruz.br

Received 29 June 2005
Accepted 19 September 2005

Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR–restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA MYCOBACTERIA) and/or sequencing analysis of the 16S–23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species.

Abbreviations: AFB, acid-fast bacillus; ITS, internal transcribed spacer; MAC, Mycobacterium avium complex; PRA, PCR–restriction enzyme analysis.






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