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J Med Microbiol 55 (2006), 1-10; DOI: 10.1099/jmm.0.46175-0
© 2006 Society for General Microbiology
ISSN 0022-2615

Distribution and genomic location of active insertion sequences in the Burkholderia cepacia complex

Dervla T. Kenna1,{dagger}, Hasan Yesilkaya2, Ken J. Forbes3, Victoria A. Barcus1, Peter Vandamme4 and John R. W. Govan1

1 Cystic Fibrosis Laboratory, Medical Microbiology, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK

2 Department of Infection, Immunity and Inflammation, University of Leicester, Maurice Shock Building, University Road, PO Box 138, Leicester LE1 9HN, UK

3 Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK

4 Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Ledeganckstraat 35, B-9000, Gent, Belgium

Correspondence
Dervla T. Kenna
dkenna{at}staffmail.ed.ac.uk

Received 30 May 2005
Accepted 30 August 2005

This study aimed firstly to establish the distribution and copy number within the Burkholderia cepacia complex of three insertion sequences (IS402, IS407 and IS1416) that possess the ability to activate transcription and hence influence gene expression. A second aim was to map the genomic insertion sites of one of the active insertion sequences (IS407) to establish putative links between insertion site and downstream gene activation. The resulting data revealed that all three insertion sequences were present in one-third of the 66 isolates tested. The three insertion sequences were prevalent across the nine B. cepacia complex species, although IS402 was absent from the 16 Burkholderia anthina strains tested and IS407 was absent from all 10 Burkholderia pyrrocinia strains. IS407 copies from six strains (two Burkholderia cenocepacia strains and one strain each of Burkholderia multivorans, Burkholderia stabilis, Burkholderia vietnamiensis and B. anthina) were mapped to the genome using hemi-nested inverse PCR. Insertions were found upstream of genes with wide-ranging functions. This study suggests that the abundance and distribution of these active insertion sequences is likely to affect genomic plasticity, and potentially gene transcription and pathogenicity.

Abbreviations: Bcc, Burkholderia cepacia complex; CF, cystic fibrosis; hINVPCR, hemi-nested inverse PCR; IS, insertion sequence.

{dagger}Present address: Cystic Fibrosis Group, Centre for Infectious Diseases, University of Edinburgh College of Medicine and Veterinary Medicine, The Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK.




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