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J Med Microbiol 54 (2005), 897-900; DOI: 10.1099/jmm.0.46029-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis

Nicasio Mancini1,2, Cristina M Ossi1, Mario Perotti1,2, Silvia Carletti1,2, Claudia Gianni1, Giorgio Paganoni3, Stanislav Matuska3, Maria Guglielminetti4, Annalisa Cavallero1, Roberto Burioni1,2, Paolo Rama3 and Massimo Clementi1,2

1,3Laboratorio di Microbiologia, Diagnostica e Ricerca San Raffaele1 and Centro Malattie della Cornea, Dipartimento di Oftalmologia e Scienze della Visione3, IRCCS Istituto Scientifico San Raffaele, via Olgettina 60, 20132 Milano, Italy 2Università ‘‘Vita-Salute San Raffaele’', Facoltà di Medicina e Chirurgia, Milano, Italy 4Dipartimento di Ecologia del Territorio e degli Ambienti Terrestri, Sezione di Micologia, Università degli Studi di Pavia, Pavia, Italy

Correspondence Nicasio Mancini nicasio.mancini{at}hsr.it

Received February 1, 2005
Accepted June 13, 2005

The present report describes the diagnostic strategy followed in a case of keratomycosis. Together with conventional methods, a molecular strategy that involved the direct sequencing of an amplified portion of the genome encompassing the internal transcribed spacer 1 and 2 regions and sequence analysis was used. The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology.

Abbreviations: b.i.d., twice per day; q.h., every hour.




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