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J Med Microbiol 54 (2005), 823-826; DOI: 10.1099/jmm.0.45868-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Identification of staphylococci by 16S internal transcribed spacer rRNA gene restriction fragment length polymorphism

Mert Sudagidan, A Fazil Yenidunya and Hatice Gunes

Department of Biology, Izmir Institute of Technology, 35430 Gulbahce, Urla, Izmir, Turkey

Correspondence Hatice Gunes haticegunes{at}iyte.edu.tr

Received August 17, 2004
Accepted May 17, 2005

The capacity of 16S internal transcribed spacer (16S-ITS) rRNA gene RFLP to differentiate 16 type strains and nine clinical isolates of staphylococci was evaluated. The 16S rRNA gene was amplified together with the ITS region and the amplification products were digested with TaqI restriction enzyme. Analysis of the 16S-ITS rRNA gene RFLP profiles differentiated each of the 16 type strains into distinct RFLP haplotypes.

Abbreviations: CNS, coagulase-negative staphylococci; ITS, internal transcribed spacer.




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