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Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Shaloo Sood¹, Praveen Rishi², Harpreet Vohra¹, Saroj Sharma² and Nirmal K Ganguly¹

¹Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India ²Department of Microbiology, Panjab University, Chandigarh 160 014, India

Correspondence Praveen Rishi rishipraveen{at}yahoo.com

Received February 10, 2005
Accepted May 18, 2005

The role of purified iron-regulated outer-membrane proteins (IROMPs) from Salmonella enterica serotype Typhi in modulation of specific T-cell responses was studied. The cellular immune response induced by IROMPs was measured by assessing the delayed-type hypersensitivity (DTH) response, lymphocyte proliferation, T-cell phenotyping and cytokine-producing cells using lymphocytes isolated from the spleen and Peyer's patches of IROMPs-immunized, immunized-challenged, infected and control mice. IROMPs immunization resulted in an enhanced DTH response and exhibited a significant increase in the protein-specific proliferative response of lymphocyte from the spleen as well as Peyer's patches. A significant increase was also observed in the ratio of CD4⁺/CD8⁺ cells in the immunized mice as compared to the infected mice. Results of the cytokine analysis revealed that during the initial period there was increased production of interleukin (IL)-2- and interferon (IFN)--producing cells in the spleen and Peyer's patches, indicating a Th1 type response, whereas in the later period of the study, increased production of IL-4-producing cells suggested a Th2 type response. The results of this study suggest a role for S. Typhi IROMPs in modulating the cellular immune response at peripheral and mucosal levels.

Abbreviations: DTH, delayed-type hypersensitivity; IFN, interferon; IL, interleukin; IROMP, iron-regulated outer-membrane protein; OMP, outer-membrane protein; PP, Peyer's patch.