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Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea

¹National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata – 700 010, India ²Jadavpur University, Jadavpur, Kolkata – 700 032, India ³Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan ⁴International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka – 1212, Bangladesh

Correspondence G. Balakrish Nair gbnair{at}icddrb.org

Received July 13, 2004
Accepted May 6, 2005

Vibrio fluvialis is a halophilic Vibrio species associated with acute diarrhoeal illness in humans. It has the potential to cause outbreaks and has an association with paediatric diarrhoea. In this study, 11 V. fluvialis strains isolated from hospitalized patients with acute diarrhoea at the Infectious Diseases Hospital, Kolkata were extensively characterized. All the strains showed growth in peptone broth containing 7 % NaCl. The strains showed variable results in Voges–Proskauer test and to a vibriostatic agent. There was also variation in their antibiograms, and some of the strains were multidrug resistant. Among the 11 strains, two showed only a single band difference in their PFGE profile and the remaining strains showed nine different PFGE patterns. However, unlike PFGE, the strains exhibited close matches and clustering in their ribotype patterns. The haemolytic effect on sheep red blood cells varied with strains. Partial sequence analysis revealed that the V. fluvialis haemolysin gene has 81 % homology with that of the El Tor haemolysin of Vibrio cholerae. A striking finding was the capability of all the strains to evoke distinct cytotoxic and vacuolation effects on HeLa cells.

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