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Phosphotidylinositol-3 kinase-mediated signals in mice immunized with the 57 kDa major antigenic outer-membrane protein of Shigella dysenteriae type 1

Division of Immunology and Vaccine Development, National Institute of Cholera & Enteric Diseases, P-33, CIT Road, Scheme: XM, Kolkata 700010, India

Correspondence A. K. Sinha ajoysinha{at}vsnl.net

Received January 13, 2005
Accepted March 15, 2005

Antigen-specific T-cell signalling via T-cell antigen receptor stimulation was carried out in BALB/c mice immunized with the 57 kDa major antigenic component of Shigella dysenteriae 1 outer-membrane proteins. In presence of anti-CD3, the 57 kDa antigen was found to increase the level of IL-2 significantly instead of IL-4. IL-2 production in T cells was consistent with an increase in intracellular free Ca²⁺ [(Ca²⁺)i] concentration. The antigen-specific modulation was observed during T-cell signalling, with enhanced release of [(Ca²⁺)i]. IL-2-receptor stimulation via IL-2 did not significantly induce the release of IL-2 with consistent intracellular Ca²⁺ production. Furthermore, the protein tyrosine kinase was activated during anti-CD3 stimulation, which up-regulated the phosphatidylinositol kinase of p85-mediated serine kinase protein kinase-C of p70. Phosphoinositide-specific kinases are regulated by the phosphorylation of tyrosine kinase through the activation of the T-cell antigen receptor. The above findings indicate that phosphotidylinositol-3 kinase-mediated signals are up-regulated through [(Ca²⁺)i], which is essential for Th1-type responses.