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J Med Microbiol 54 (2005), 539-542; DOI: 10.1099/jmm.0.45599-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Diagnosis of partially treated culture-negative bacterial meningitis using 16S rRNA universal primers and restriction endonuclease digestion

Lekha Pandit1, Sanath Kumar2, Indrani Karunasagar2 and Iddya Karunasagar2

1Department of Neurology, KS Hegde Medical Academy, Deralakatte, Mangalore, India 2Department of Fisheries Microbiology, University of Agricultural Sciences, Mangalore, India

Correspondence Iddya Karunasagar mircen{at}sancharnet.in

Received January 15, 2004
Accepted February 5, 2005

Cerebrospinal fluid (CSF) obtained from patients with partially treated and culture-negative meningitis was subjected to PCR using 16S rDNA universal primers followed by restriction endonuclease digestion. In all, 43 patients and 7 controls were enrolled in this study. Twenty-one meningitic samples were positive by PCR. Mycobacterium tuberculosis was the causative agent in seven cases followed by Haemophilus influenzae (four), Streptococcus pneumoniae (two), Listeria monocytogenes (one), Escherichia coli (one), Pseudomonas aeruginosa (one) and Staphylococcus aureus (one). Only two meningitic CSF samples were culture-positive. In this study, PCR using bacterial 16S rDNA specific universal primers was found to be superior to conventional methods in the diagnosis of partially treated meningitis.

Abbreviation: CSF, cerebrospinal fluid.




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L.-H. Chen, Q.-J. Duan, M.-T. Cai, Y.-D. Wu, and S.-Q. Shang
Rapid Diagnosis of Sepsis and Bacterial Meningitis in Children with Real-Time Fluorescent Quantitative Polymerase Chain Reaction Amplification in the Bacterial 16S rRNA Gene
Clinical Pediatrics, July 1, 2009; 48(6): 641 - 647.
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