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Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis

Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK

Correspondence Craig Winstanley C.Winstanley{at}liv.ac.uk

Received January 4, 2005
Accepted February 18, 2005

A collection of 63 isolates of Pseudomonas aeruginosa associated with ulcerative keratitis, collected from six centres in England, were typed using serotyping and random amplified polymorphic DNA-PCR, and screened for several variable virulence-related genotypes and phenotypes. Sixty-one percent of the isolates were of either serotype O1 or serotype O11, but there was no evidence for a common clone. The majority of isolates (59 %) were PCR-positive for exoU rather than for exoS (38 %), and carried a-type fliC genes (76 %) rather than b-type (24 %). Isolates were PCR-positive for pyoverdine-receptor types at a prevalence of 38 % for type I, 46 % for type II and 8 % for type III. All but one of the isolates exhibited twitching activity. There was a correlation between the presence of exoS and twitching activity (P = 0.04), suggesting that a combination of exoS genotype and good twitching activity may have a role to play in ExoU-independent corneal virulence.

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