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J Med Microbiol 54 (2005), 481-483; DOI: 10.1099/jmm.0.45880-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods

Lyudmila Boyanova1, Galina Gergova1, Rossen Nikolov2, Sirigan Derejian2, Elena Lazarova3, Nikolai Katsarov4, Ivan Mitov1 and Zacharii Krastev2

1Department of Microbiology, Medical University of Sofia, Zdrave Street 2, 1431 Sofia, Bulgaria 2Department of Gastroenterology, St Ivan Rilski Hospital, Bulgaria 3Department of Gastroenterology, Pediatric Hospital, Bulgaria 4Alexander Hospital, Second Surgery Hospital, Sofia, Bulgaria

Correspondence Lyudmila Boyanova lboyanova{at}hotmail.com

Received August 26, 2004
Accepted January 24, 2005

Propolis exhibits antimicrobial, anti-inflammatory and other biological effects. The aim of this study was to evaluate the activity of 30 % ethanolic extract of Bulgarian propolis against 94 Helicobacter pylori strains by three methods. By the agar-well diffusion method, only 13.8 % of the strains exhibited no inhibition by 30 µl propolis extract (containing 9 mg propolis) and all isolates were inhibited to some extent by 90 µl of the extract (27 mg propolis) per well. The mean diameters of growth inhibition by 30, 60 or 90 µl propolis extract or 30 µl 96 % ethanol per well were 16.8, 19.2, 27.5 and 8.3 mm, respectively. The propolis extract was more active than the ethanol (P < 0.001). With 90 µl propolis extract per well, 69.4 % of the strains exhibited large diameters of growth inhibition (>=20 mm) versus 26.6 % with 30 µl per well (P < 0.001). With moist propolis discs, inhibition was detected in more strains (92.1 %) than with dried discs (78.2 %, P < 0.05), with mean inhibitory diameters of 18.7 and 13.8 mm, respectively. By the agar dilution method, 100 and 300 µg propolis ml–1 inhibited the growth of 57.1 % and 76.2 %, respectively, of the 21 strains tested. In conclusion, Bulgarian propolis had a strong and dose-dependent activity against most of the H. pylori strains tested. Although the effect of propolis on H. pylori in vitro is promising, further microbiological, pharmacological and clinical trials are required.






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