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Failure to detect capsule gene bexA in Haemophilus influenzae types e and f by real-time PCR due to sequence variation within probe binding sites

Health Protection Agency London, Department of Virology, King's College Hospital (Dulwich site), Dulwich Hospital, East Dulwich Grove, London SE22 8QF, UK

Correspondence M. Smith melvyn.smith{at}kcl.ac.uk

Received July 23, 2004
Accepted January 21, 2005

Detection of the conserved capsule gene bexA is used to distinguish capsulate from non-capsulate Haemophilus influenzae. While developing a real-time PCR assay to detect bexA, it was found that bexA probes produced a detectable signal for H. influenzae types a to d, but failed to do so for H. influenzae types e and f. Sequencing revealed differences compared with H. influenzae types a to d within probe binding sites. To prevent misclassification of strains as non-capsulate, assays must detect all capsular types.

Sequence alignment data are available as supplementary data in JMM Online.

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