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J Med Microbiol 54 (2005), 375-379; DOI: 10.1099/jmm.0.45748-0
© 2005 Society for General Microbiology
ISSN 0022-2615

A fraction from Escherichia coli with anti-Aspergillus properties

V Yadav1, R Mandhan2, Rajesh Dabur3, A K Chhillar1, J Gupta1 and G L Sharma1,3

1Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi, India 2Department of Biotechnology, Kurukshetra University, Kurukshetra, India 3Department of Biomedical Sciences, Bundelkhund University, Jhansi, India

Correspondence G. L. Sharma drglsharma{at}hotmail.com

Received May 18, 2004
Accepted December 2, 2004

The products of various strains of Escherichia coli (BL21, DH5{alpha}, HB101 and XL Blue) were investigated for antimycotic properties using pathogenic isolates of Aspergillus. Co-culture experiments revealed that E. coli strains exhibited variable activity against Aspergillus fumigatus. The lysates prepared from DH5{alpha}, HB101 and XL Blue strains of E. coli showed inhibitory activity against A. fumigatus in the protein concentration range of 62.50 to 250.00 µg ml–1. The highest activity was seen in the lysate of BL21, which inhibited the growth of A. fumigatus and Aspergillus flavus completely at a concentration of 31.25 µg protein ml–1. The MIC of BL21 lysate against Aspergillus niger was found to be 62.50 µg ml–1. The in vitro toxicity of BL21 lysate was evaluated using a haemolytic assay. A BL21 lysate protein concentration of 1250.00 µg ml–1 was found to be nontoxic to human erythrocytes. The standard drug amphotericin B lysed 100 % of erythrocytes at a concentration of 37.50 µg ml–1. SDS-PAGE showed the presence of at least 15 major proteins in the lysate of BL21. Ion-exchange chromatography resolved the BL21 lysate into five fractions and fraction III was found to be endowed with anti-Aspergillus properties. The MIC of this fraction was found to be 3.90 µg ml–1. Further work on the purification of the active molecule and its characterization is in progress.




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V. Yadav, R. Mandhan, Q. Pasha, S. Pasha, A. Katyal, A. K. Chhillar, J. Gupta, R. Dabur, and G. L. Sharma
An antifungal protein from Escherichia coli
J. Med. Microbiol., May 1, 2007; 56(5): 637 - 644.
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