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J Med Microbiol 54 (2005), 357-360; DOI: 10.1099/jmm.0.45911-0
© 2005 Society for General Microbiology
ISSN 0022-2615

In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies

Yurika Ikeda-Dantsuji, Ichiro Konomi and Ariaki Nagayama

Department of Microbiology and Immunology, School of Medicine, Fukuoka University, 7-45-1, Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan

Correspondence Ariaki Nagayama nagayama{at}fukuoka-u.ac.jp

Received September 30, 2004
Accepted December 6, 2004

The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis. The number of inclusions of C. trachomatis at the end point of the APTIMA Combo 2 assay was 0.005 inclusion-forming units (i.f.u.) ml–1, which was equivalent to 0.008 EBs per assay. The end point of the AMPLICOR kit was 5 i.f.u. ml–1 (equivalent to 0.5 EB per assay). The efficacy of the AMPLICOR C. trachomatis assay was inhibited by phosphate or Fe2+ ion, while these had no effect on the APTIMA Combo 2 assay. In conclusion, the APTIMA Combo 2 assay appears to have a greater sensitivity than the AMPLICOR C. trachomatis assay for detection of C. trachomatis, while demonstrating few problems with inhibitory substances such as phosphate and Fe2+ ion.

Abbreviations: EB, elementary body; i.f.u., inclusion-forming unit; NAA, nucleic acid amplification.




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