J Med Microbiol NEW Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gladstone, P.
Right arrow Articles by Brahmadathan, K N
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gladstone, P.
Right arrow Articles by Brahmadathan, K N
Agricola
Right arrow Articles by Gladstone, P.
Right arrow Articles by Brahmadathan, K N
J Med Microbiol 54 (2005), 305-308; DOI: 10.1099/jmm.0.45555-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Dynamics of anti-M antibody response in a mouse model following intranasal infection with group A Streptococcus M-18

Paul Gladstone1, George Varghese2 and K N Brahmadathan1

1Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore – 632004, Tamil Nadu, India 2Department of Microbiology, Pushpagiri Medical College, Tiruvalla – 689 101, Kerala, India

Correspondence K. N. Brahmadathan knb1948{at}hotmail.com

Received November 28, 2003
Accepted November 8, 2004

Dynamics of anti-M antibody response following intranasal infection with group A Streptococcus (GAS) M-18 were investigated in a Swiss albino mouse model. Mice arranged in three groups were inoculated intranasally with 2.0 x 107 c.f.u. ml–1 of GAS M-18 on 1, 2 alternate and 3 alternate days. Plasma collected from the retro-orbital plexus was tested for antibodies by an in-house indirect ELISA. The antibody titres of the plasma samples varied from 1 : 8 to 1 : 1024 in the 1 day dose, from 1 : 4 to 1 : 256 in the 2 day dose and from 1 : 4 to 1 : 128 in the 3 day dose. Peak titres were seen on day 42 or 56 and in all cases the titres had declined by day 84. Swiss albino mouse can thus serve as a useful animal model to study different aspects of type-specific anti-M immune responses against GAS disease when designing candidate streptococcal vaccines.

Abbreviation: GAS, group A streptococci.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2005 Society for General Microbiology.