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J Med Microbiol 54 (2005), 273-278; DOI: 10.1099/jmm.0.45908-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Molecular characterization of a multidrug-resistant strain of enteroinvasive Escherichia coli O164 isolated in Japan

Ashraf M Ahmed1, Shin-ichi Miyoshi2, Sumio Shinoda2 and Tadashi Shimamoto1

1Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan 2Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan

Correspondence Tadashi Shimamoto tadashis{at}hiroshima-u.ac.jp

Received September 27, 2004
Accepted November 26, 2004

Enteroinvasive Escherichia coli (EIEC) O164 strain RIMD05091045 was isolated from a travelling patient suffering from diarrhoea at the Osaka airport quarantine facility in Japan. The strain showed multidrug resistance against streptomycin, spectinomycin, co-trimoxazole (trimethoprim/sulfamethoxazole) and ampicillin, and reduced susceptibility to ciprofloxacin. Molecular characterization of the multidrug-resistance phenotype revealed the presence of a class 1 integron containing three genes, a dihydrofolate reductase type XII gene, dfrXII, which confers resistance to trimethoprim, an aminoglycoside adenyltransferase gene, aadA2, which confers resistance to streptomycin and spectinomycin, and an ORF of unknown function. Southern blot hybridization and conjugation experiments showed that the class 1 integron was located on a transferable plasmid that was less than 90 kb in size. The resistance of EIEC O164 to ampicillin was found to be due to the presence of TEM-1 ß-lactamase. On the other hand, a single mutation that has not previously been described, P158-to-S, was detected downstream of the quinolone-resistance-determining region of parC of topoisomerase IV and may be responsible for the reduced susceptibility to ciprofloxacin in this strain.

Abbreviations: CCCP, carbonyl cyanide m-chlorophenylhydrazone; EIEC, enteroinvasive Escherichia coli; ETEC, enterotoxigenic Escherichia coli; MDR, multidrug resistance; QRDR, quinolone resistance-determining region.

The GenBank/EMBL/DDBJ accession numbers for the class 1 integron sequence, including the dfrXII and aadA2 genes and an unknown ORF, and the blaTEM-1 gene sequence of EIEC O164 strain RIMD05091045 are AB154407 and AB194682, respectively.




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A. M Ahmed, H. Kawamoto, K. Inouye, Y. Hashiwata, M. Sakaki, M. Seno, and T. Shimamoto
Genomic analysis of a multidrug-resistant strain of enterohaemorrhagic Escherichia coli O157 : H7 causing a family outbreak in Japan
J. Med. Microbiol., September 1, 2005; 54(9): 867 - 872.
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