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J Med Microbiol 54 (2005), 159-162; DOI: 10.1099/jmm.0.45809-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Toxigenic status of Clostridium difficile in a large Spanish teaching hospital

R Alonso, A Martín, T Peláez, M Marín, M Rodríguez-Creixéms and E Bouza

Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ‘‘Gregorio Marañón’', C/Doctor Esquerdo, 46, 28007 Madrid, Spain

Correspondence R. Alonso ralonso.hgugm{at}salud.madrid.org

Received July 2, 2004
Accepted September 27, 2004

The aim of this study was to evaluate the toxigenic status of circulating strains of Clostridium difficile in a large teaching hospital. Overall 220 isolates were studied of which 199 (90.5 %) produced both large clostridial toxins detected by conventional methods. Ten more strains (4.5 %) had toxin A and B genes detectable by PCR. Eleven (5.0 %) variant strains (A–B+) were detected among the isolates studied and 10 strains (4.5 %) had the binary toxin genes (cdtA and cdtB).

This paper was presented at the First International Clostridium difficile Symposium, Kranjska Gora, Slovenia, 5–7 May 2004.

Abbreviation: LCTs, large clostridial toxins.




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