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J Med Microbiol 54 (2005), 155-157; DOI: 10.1099/jmm.0.45808-0
© 2005 Society for General Microbiology
ISSN 0022-2615

An improved protocol for pulsed-field gel electrophoresis typing of Clostridium difficile

R Alonso, A Martín, T Peláez, M Marín, M Rodríguez-Creixéms and E Bouza

Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario ‘‘Gregorio Marañón’', C/Doctor Esquerdo, 46, 28007 Madrid, Spain

Correspondence R. Alonso ralonso.hgugm{at}salud.madrid.org

Received July 2, 2004
Accepted October 24, 2004

Pulsed-field gel electrophoresis (PFGE) is the ‘gold standard’ technique for bacterial typing and has proved to be discriminatory and reproducible for typing Clostridium difficile. Nevertheless, a high proportion of strains are non-typable by this technique due to the degradation of the DNA during the process. The introduction of several modifications in the PFGE standard procedure increased typability from 40 % (90 isolates) to 100 % (220 isolates) while maintaining the high degree of discrimination and reproducibility of the technique.

This paper was presented at the First International Clostridium difficile Symposium, Kranjska Gora, Slovenia, 5–7 May 2004.




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