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J Med Microbiol 54 (2005), 137-141; DOI: 10.1099/jmm.0.45790-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Generation of an erythromycin-sensitive derivative of Clostridium difficile strain 630 (630{Delta}erm) and demonstration that the conjugative transposon Tn916{Delta}E enters the genome of this strain at multiple sites

Haitham A Hussain, Adam P Roberts and Peter Mullany

Division of Microbial Diseases, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK

Correspondence Peter Mullany pmullany{at}eastman.ucl.ac.uk

Received June 24, 2004
Accepted August 11, 2004

Erythromycin resistance in Clostridium difficile strain 630 is conferred by a genetic element termed Tn5398 which contains two erm(B) genes: erm1(B) and erm2(B). An erythromycin-sensitive derivative of strain 630 (designated 630{Delta}erm) was generated by spontaneous mutation after continuous subculture for 30 days. This strain had lost the erm2(B) gene from within Tn5398 but retained erm1(B). However, the strain could revert to erythromycin resistance at a frequency of 2.79 x 10–8, although it still contained the deletion of erm2(B). The availability of C. difficile 630{Delta}erm allowed the behaviour of Tn916{Delta}E to be investigated in this strain. This element entered the genome at multiple sites indicating that it could be useful as an insertional mutagen.

This paper was presented at the First International Clostridium difficile Symposium, Kranjska Gora, Slovenia, 5–7 May 2004.

Abbreviation: CDAD, Clostridium difficile-associated disease.




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J. Med. Microbiol., February 1, 2005; 54(2): 97 - 100.
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