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J Med Microbiol 54 (2005), 1213-1216; DOI: 10.1099/jmm.0.45909-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Comparison of three Legionella urinary antigen assays during an outbreak of legionellosis in Belgium

Kristien Dirven1{dagger}, Margareta Ieven2, Marcel F Peeters3, Anneke van der Zee3, Koen De Schrijver4 and Herman Goossens2

1Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium

Correspondence Kristien Dirven kristien.dirven{at}ua.ac.be

Received 28 September 2004
Accepted 22 August 2005

During an outbreak of legionellosis in Belgium, urine samples of 32 legionellosis patients were tested with three Legionella urinary antigen assays: the Biotest enzyme immunoassay (EIA) kit, the Binax EIA kit and the Binax NOW Immunochromatographic Test kit. The three tests were concomitantly compared. The test sensitivities on the first urine samples were 65.6 % for the Biotest EIA, 50.0 % for the Binax EIA and 56.3 % for the Binax NOW. Testing of a second urine sample increased the sensitivities to 71.9 %, 59.4 % and 65.6 %, respectively. The differences were not statistically significant. In outbreak settings, testing second samples from patients presenting with symptoms but initially testing negative and/or concentrating urine samples for testing might be valuable additions to the urinary antigen test to increase the sensitivities of the tests.

{dagger}Present address: Center for General Practice, Building S, Room S5.40, University of Antwerp UA, Universiteitsplein 1, B-2610, Wilrijk, Belgium.

Abbreviations: EIA, enzyme immunoassay; ICT, immunochromatographic test.






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