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J Med Microbiol 54 (2005), 1183-1187; DOI: 10.1099/jmm.0.46160-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Rapid and simple detection of blaCTX–M genes by multiplex PCR assay

Li Xu1,2, Vicki Ensor2, Savita Gossain1, Kathy Nye1 and Peter Hawkey1,2

1Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK 2Division of Immunity and Infection, University of Birmingham, UK

Correspondence Li Xu li.xu{at}heartofengland.nhs.uk

Received 18 May 2005
Accepted 5 August 2005

A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of blaCTX–M genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified blaCTX–M genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

Abbreviations: DHPLC, denaturing HPLC; ESBL, extended-spectrum ß-lactamase.




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