J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertrand, S.
Right arrow Articles by Collard, J.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertrand, S.
Right arrow Articles by Collard, J.-M.
Agricola
Right arrow Articles by Bertrand, S.
Right arrow Articles by Collard, J.-M.
J Med Microbiol 54 (2005), 1151-1156; DOI: 10.1099/jmm.0.46142-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Detection and characterization of tet(M) in tetracycline-resistant Listeria strains from human and food-processing origins in Belgium and France

Sophie Bertrand1, Geert Huys2, Marc Yde1, Klaas D'Haene2, Florence Tardy3, Martine Vrints1, Jean Swings2,4 and Jean-Marc Collard1

1Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France

Correspondence Sophie Bertrand s.bertrand{at}iph.fgov.be

Received 29 April 2005
Accepted 3 August 2005

In the present study, three Listeria monocytogenes strains and one Listeria innocua strain out of a collection of 241 Listeria isolates from human and food-processing sources were found to display resistance to tetracycline (TC) due to the presence of the tet(M) gene. Through sequence analysis, it was shown that tet(M) genes in two of the isolates belong to sequence homology group (SHG) II, a group comprising chromosomally encoded tet(M) genes previously found in Staphylococcus aureus and in lactobacilli. The tet(M) genes of the two other L. monocytogenes strains were associated with a member of the Tn916–Tn1545 family of conjugative transposons and were closely related to SHG III, which harbours enterococcal tet(M) genes associated with Tn916. One of these transposon-containing strains was able to transfer the tet(M) gene to Enterococcus faecalis recipient strain JH2-2. Collectively, these sequence and conjugation data indicate that the acquisition of tet(M) by Listeria strains may be triggered by successive transfers between other Gram-positive organisms.

Abbreviations: MC, minocycline; TC, tetracycline.

The GenBank/EMBL/DDBJ accession numbers for the partial sequences of the tet(M) genes of four Listeria strains described in this study are AJ704565–AJ704568.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2005 Society for General Microbiology.