J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L
Agricola
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L
J Med Microbiol 54 (2005), 1133-1138; DOI: 10.1099/jmm.0.46244-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization

Fanrong Kong1, Lin Ma1,2 and Gwendolyn L Gilbert1

1Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales, Australia 2Department of Dermatology, Beijing Children's Hospital, Affiliate of Capital University of Medical Sciences, Beijing, PR China

Correspondence Gwendolyn L. Gilbert lyng{at}icpmr.wsahs.nsw.gov.au

Received 14 July 2005
Accepted 12 August 2005

Streptococcus agalactiae (group B streptococcus, GBS) is an important cause of sepsis in neonates and their mothers, and the elderly and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution is needed to guide the development and assess the feasibility of GBS conjugate vaccines. The authors previously developed a molecular serotype identification method based on serotype-specific PCR and partial sequencing of cps genes. In this study, a novel 10-primer pair multiplex PCR and reverse line blot (mPCR/RLB) hybridization assay was developed for simultaneous detection and serotype identification of all nine GBS serotypes. For all 316 GBS isolates tested the mPCR/RLB results corresponded with those of conventional serotyping and individual serotype-specific PCR, and the method was more convenient and practical than either alternative.

Abbreviations: CS, conventional serotype/ing; GBS, group B streptococcus; mPCR, multiplex PCR; MS, molecular serotype/ing; RLB, reverse line blot.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
L. Cai, F. Kong, Q. Wang, H. Wang, M. Xiao, V. Sintchenko, and G. L. Gilbert
A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing
J. Med. Microbiol., August 1, 2009; 58(8): 1045 - 1057.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. B. Ulett, W. H. Benjamin Jr., F. Zhuo, M. Xiao, F. Kong, G. L. Gilbert, M. A. Schembri, and G. C. Ulett
Diversity of Group B Streptococcus Serotypes Causing Urinary Tract Infection in Adults
J. Clin. Microbiol., July 1, 2009; 47(7): 2055 - 2060.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. Kong, L. M. Lambertsen, H.-C. Slotved, D. Ko, H. Wang, and G. L. Gilbert
Use of Phenotypic and Molecular Serotype Identification Methods To Characterize Previously Nonserotypeable Group B Streptococci
J. Clin. Microbiol., August 1, 2008; 46(8): 2745 - 2750.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H.-C. Slotved, F. Kong, L. Lambertsen, S. Sauer, and G. L. Gilbert
Serotype IX, a Proposed New Streptococcus agalactiae Serotype
J. Clin. Microbiol., September 1, 2007; 45(9): 2929 - 2936.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
C. Poyart, A. Tazi, H. Reglier-Poupet, A. Billoet, N. Tavares, J. Raymond, and P. Trieu-Cuot
Multiplex PCR Assay for Rapid and Accurate Capsular Typing of Group B Streptococci
J. Clin. Microbiol., June 1, 2007; 45(6): 1985 - 1988.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
X. Zeng, F. Kong, J. Morgan, and G. L. Gilbert
Evaluation of a Multiplex PCR-Based Reverse Line Blot-Hybridization Assay for Identification of Serotype and Surface Protein Antigens of Streptococcus agalactiae.
J. Clin. Microbiol., October 1, 2006; 44(10): 3822 - 3825.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
F. Kong, H. F. Gidding, R. Berner, and G. L. Gilbert
Streptococcus agalactiae C{beta} protein gene (bac) sequence types, based on the repeated region of the cell-wall-spanning domain: relationship to virulence and a proposed standardized nomenclature.
J. Med. Microbiol., July 1, 2006; 55(Pt 7): 829 - 837.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. Kong, M. Brown, A. Sabananthan, X. Zeng, and G. L. Gilbert
Multiplex PCR-Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes.
J. Clin. Microbiol., May 1, 2006; 44(5): 1887 - 1891.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
L. Wen, Q. Wang, Y. Li, F. Kong, G. L. Gilbert, B. Cao, L. Wang, and L. Feng
Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus (Streptococcus agalactiae)
J. Clin. Microbiol., April 1, 2006; 44(4): 1447 - 1452.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2005 Society for General Microbiology.